School of Sciences and Humanities
School of Sciences and Humanities
School of Sciences and Humanities
Department of Biology
List of Department Biology Research laboratories
To be filled
Timo Burster
Laboratory: C4, 610
Email: timo.burster@nu.edu.kz
1. Research
1. 1. The cooperation between proteases and host cells is pivotal in mounting an effective innate immune response. Such an immune response is initiated, in particular, by neutrophils releasing granules containing serine proteases, including neutrophil elastase (NE), cathepsin G (CatG), protease 3 (PR3), and neutrophil serine protease 4 (NSP4), collectively classified as neutrophil serine proteases (NSPs). Functional responsibilities of NSPs, specifically bivalent regulation of major histocompatibility complex class I (MHC) molecules, underscore a novel role of NSPs within the immune system. Furthermore, we are interested in generating and applying novel activity-based probes for activity-based protein profiling using Western blot, SDS-PAGE, fluorescence microscopy, and flow cytometry to detect proteolytic active serine protease and test novel serine protease inhibitors. The additional part of the research includes antigen processing of the S protein of SARS-CoV-2 and their novel variants and subvariants. Finally, several proteome profiler arrays were established in the laboratory in order to determine protease as well as protease inhibitors.
1.2. The synergistic interaction of CatG with the human alveolar basal epithelial cell line is of particular interest due to its potential implications against viral infection. Our study aimed to investigate the binding capacity of CatG on the surface of such cells. CatG shows proteolytic activity to fortify target cells against viral entrance.
1.3. Perspectives: Investigating the regulation of mediators in asymptomatic- and symptomatic patients as well as individuals with long COVID-19.
2. Selected publications:
2.1. Zhadyra Mustafa, Hubert Kalbacher, and Timo Burster. Occurrence of a novel cleavage site for cathepsin G adjacent to the polybasic sequence within the proteolytically sensitive activation loop of the SARS-CoV-2 Omicron variant: The amino acid substitution N679K and P681H of the spike protein. 2022. PlosOne. 10.1371/journal.pone.0264723. IF: 3.752. SJR: 0.85, Q1
2.2. Akmaral Assylbekova, Anuar Zhanapiya, Renata Grzywa, Marcin Sienczyk, Christian Schönbach and Timo Burster. Camostat Does Not Inhibit the Proteolytic Activity of Neutrophil Serine Proteases. 2022. Pharmaceuticals. IF: 5.215. SJR: 0.85, Q1
2.3. Nadine Schilling, Hubert Kalbacher, Timo Burster. Variation of proteolytic cleavage sites towards the N-terminal end of the S2 subunit of the novel SARS-CoV-2 Omicron sublineage BA.2.12.1. 2022. Molecules. IF: 4.927. SJR: 0.71, Q1
Laboratory: C4, 610
Email: timo.burster@nu.edu.kz
1. Research
1. 1. The cooperation between proteases and host cells is pivotal in mounting an effective innate immune response. Such an immune response is initiated, in particular, by neutrophils releasing granules containing serine proteases, including neutrophil elastase (NE), cathepsin G (CatG), protease 3 (PR3), and neutrophil serine protease 4 (NSP4), collectively classified as neutrophil serine proteases (NSPs). Functional responsibilities of NSPs, specifically bivalent regulation of major histocompatibility complex class I (MHC) molecules, underscore a novel role of NSPs within the immune system. Furthermore, we are interested in generating and applying novel activity-based probes for activity-based protein profiling using Western blot, SDS-PAGE, fluorescence microscopy, and flow cytometry to detect proteolytic active serine protease and test novel serine protease inhibitors. The additional part of the research includes antigen processing of the S protein of SARS-CoV-2 and their novel variants and subvariants. Finally, several proteome profiler arrays were established in the laboratory in order to determine protease as well as protease inhibitors.
1.2. The synergistic interaction of CatG with the human alveolar basal epithelial cell line is of particular interest due to its potential implications against viral infection. Our study aimed to investigate the binding capacity of CatG on the surface of such cells. CatG shows proteolytic activity to fortify target cells against viral entrance.
1.3. Perspectives: Investigating the regulation of mediators in asymptomatic- and symptomatic patients as well as individuals with long COVID-19.
2. Selected publications:
2.1. Zhadyra Mustafa, Hubert Kalbacher, and Timo Burster. Occurrence of a novel cleavage site for cathepsin G adjacent to the polybasic sequence within the proteolytically sensitive activation loop of the SARS-CoV-2 Omicron variant: The amino acid substitution N679K and P681H of the spike protein. 2022. PlosOne. 10.1371/journal.pone.0264723. IF: 3.752. SJR: 0.85, Q1
2.2. Akmaral Assylbekova, Anuar Zhanapiya, Renata Grzywa, Marcin Sienczyk, Christian Schönbach and Timo Burster. Camostat Does Not Inhibit the Proteolytic Activity of Neutrophil Serine Proteases. 2022. Pharmaceuticals. IF: 5.215. SJR: 0.85, Q1
2.3. Nadine Schilling, Hubert Kalbacher, Timo Burster. Variation of proteolytic cleavage sites towards the N-terminal end of the S2 subunit of the novel SARS-CoV-2 Omicron sublineage BA.2.12.1. 2022. Molecules. IF: 4.927. SJR: 0.71, Q1
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Soltabayeva Laboratory (Stressed plant physiology)
Location: C4 649
Major Research Directions:
1. Investigation of the effects of environmental factors (heat, drought and heavy metal) on plant stress responses and senescence in the model plant Arabidopsis thaliana and crops such as wheat and barley.
2. Study of senescence markers, including morphological, physiological, and molecular indicators, in Pinus sylvestris.
3. Early detection of potato spoilage in agricultural storage.
Selected Publications :
A. Kurmanbayeva, A. Soltabayeva*, et al. "Investigating the Role of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK) in Senescence Regulation and Developmental Processes in Arabidopsis." Acta Physiologiae Plantarum, 2024.
A. Soltabayeva, A. Kurmanbayeva, et al. "Endogenous ureides are employed as a carbon source in Arabidopsis plants exposed to carbon starvation conditions." Plant Science, 2024, Volume 344, 112108.
A. Soltabayeva and M. Sagi "Determination of ROS Generated by Arabidopsis Xanthine Dehydrogenase1 (AtXDH1) Using Nitroblue Tetrazolium (NBT) and 3,3′-Diaminobenzidine (DAP)", Springer Nature 2024, Chapter 5, ,Vol. 2798 Internal Collaborations: Biocluster, SEDS, NLA.
External Collaborations:
International Science Complex “Astana” - Project on "Restoration and preservation of the ecosystem of the State National Park 'Burabay'", SPUN (Society for the Protection of Underground Networks) https://spun-test.webflow.io/expeditions/kazakhstan
Location: C4 649
Major Research Directions:
1. Investigation of the effects of environmental factors (heat, drought and heavy metal) on plant stress responses and senescence in the model plant Arabidopsis thaliana and crops such as wheat and barley.
2. Study of senescence markers, including morphological, physiological, and molecular indicators, in Pinus sylvestris.
3. Early detection of potato spoilage in agricultural storage.
Selected Publications :
A. Kurmanbayeva, A. Soltabayeva*, et al. "Investigating the Role of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK) in Senescence Regulation and Developmental Processes in Arabidopsis." Acta Physiologiae Plantarum, 2024.
A. Soltabayeva, A. Kurmanbayeva, et al. "Endogenous ureides are employed as a carbon source in Arabidopsis plants exposed to carbon starvation conditions." Plant Science, 2024, Volume 344, 112108.
A. Soltabayeva and M. Sagi "Determination of ROS Generated by Arabidopsis Xanthine Dehydrogenase1 (AtXDH1) Using Nitroblue Tetrazolium (NBT) and 3,3′-Diaminobenzidine (DAP)", Springer Nature 2024, Chapter 5, ,Vol. 2798 Internal Collaborations: Biocluster, SEDS, NLA.
External Collaborations:
International Science Complex “Astana” - Project on "Restoration and preservation of the ecosystem of the State National Park 'Burabay'", SPUN (Society for the Protection of Underground Networks) https://spun-test.webflow.io/expeditions/kazakhstan
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