Prof. Dyab in collaboration with Prof. Paunov’s research group developed new platform for formation of 3D cell networks in capillary suspensions of Human cells based on aqueous two-phase systems.

The article presents a novel method for fabricating three-dimensional (3D) structured human cell networks using capillary cell suspensions based on aqueous two-phase systems (ATPS). By combining dextran (DEX) and polyethylene glycol (PEG) aqueous phases, we formed water-in-water capillary bridges that connect adherent HeLa cells into stable 3D networks. The study demonstrates that adding 2% DEX to a concentrated HeLa cell suspension in PEG yields optimal rheological and structural properties, forming robust, scaffold-free tissue-like materials without compromising cell viability. This capillary suspension approach overcomes limitations of traditional spheroid cultures, such as necrotic core formation and offers a promising, cost-effective platform for 3D cell culture and tissue engineering.

This approach introduces capillary forces in an aqueous two-phase system (PEG–DEX) as a mechanism for building stable 3D networks of adherent cells. Unlike traditional techniques, it balances structural integrity and cell health without relying on synthetic matrices or causing necrotic core formation.

The capillary suspension method bridges the gap between physiological relevance and practical usability in 3D culture. It retains the high fidelity of scaffold-free models while enhancing structural control and reducing limitations like necrosis and material interference, offering a promising new avenue for tissue engineering and biomedical research.

To read the full paper, click this link.

Paunov research group website: https://paunovgroup.org/publications-2/